Moist heat sterilization simple notes:
To above 100°c.
Saturated steam under pressure.
Steam sterilization - autoclave - Chamberland in 1884 hot saturated steam continues to enter the chamber reaches desired to and pressure 121°c for 15lbs.
It destroys all vegetative cells and spore in 10 - 12 minutes.
It effectively kill microorganisms by degrading nucleic acid and denaturing enzyme and proteins.
Distribution cell membranes.
Extended sterilization - 121°C at 15lbs for 70 minutes.
Biological indicator is used to for check the sterility of autoclave.
After autoclaving,the ampule is aseptically broken and the culture incubated for several days.
If the test organism does not grow in the medium, the sterilization run has been successful.
Sometimes either special taps that spells the word sterile or change in the color of the paper stripe represents the autoclave is sterile.
The method is convenient and save time.
To above 100°c.
Saturated steam under pressure.
Steam sterilization - autoclave - Chamberland in 1884 hot saturated steam continues to enter the chamber reaches desired to and pressure 121°c for 15lbs.
It destroys all vegetative cells and spore in 10 - 12 minutes.
It effectively kill microorganisms by degrading nucleic acid and denaturing enzyme and proteins.
Distribution cell membranes.
Extended sterilization - 121°C at 15lbs for 70 minutes.
Biological indicator is used to for check the sterility of autoclave.
After autoclaving,the ampule is aseptically broken and the culture incubated for several days.
If the test organism does not grow in the medium, the sterilization run has been successful.
Sometimes either special taps that spells the word sterile or change in the color of the paper stripe represents the autoclave is sterile.
The method is convenient and save time.
No comments:
Post a Comment