Dark field microscope;
Produce a dark background against which objects are bright illuminated.
Uses a special kind of condenser called annular stop transmits a hollow cone of light from the source of illumination.
Light directed through the condenser does not enter the objective field is dark.
Some of the light rays will be scattered or diffracted.
Diffracted light will enter the objective and reach the eye.
Object appear bright against background.
Applications :
For examination of unstained microorganisms went mount hanging drop method.
Produce a dark background against which objects are bright illuminated.
Uses a special kind of condenser called annular stop transmits a hollow cone of light from the source of illumination.
Light directed through the condenser does not enter the objective field is dark.
Some of the light rays will be scattered or diffracted.
Diffracted light will enter the objective and reach the eye.
Object appear bright against background.
Applications :
For examination of unstained microorganisms went mount hanging drop method.
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